Photosynthesis experiment. See description for explanation.
Photosynthesis experiment. Each tube (cuvette) contains a spinach chloroplast suspension that has been mixed with the chemical DPIP which acts as an electron receptor for the light reactions of photosynthesis. As it receives electrons (reduction) it progressively turns from blue to colorless. Every 5 minutes the tubes are placed in a spectrophotometer and the light transmittance is measured. The rate of photosynthesis is thus measured by an increase in light tarnsmittance in the tubes in which DPIP is changing color. Tube #1 is a blank for calibrating the spectrophotometer. Tube #2 is a tube enclosed in foil to prevent light from hitting th chloroplasts. Tube #3 is live chloroplasts in the light. Tube #4 is boiled (denatured) chloroplasts in the light. The last tube is a control containing only DPIP and no chloroplasts. See images BWF8AJ and BWDKF4 for the spectrophotometer being used.
Size: 5146px × 3417px
Photo credit: © MShieldsPhotos / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: #4, 4, advanced, ap, biology, chloroplast, chloroplasts, class, college, contolled, controlled, cuvette, cuvettes, dark, experiment, high, investigation, lab, level, light, photosynthesis, placement, school, science, set, set-, suspension, suspsension, test, tubes, variable, variables
