. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Quinone-imine Dyes 123 H r I I -I \ Q 4-. CO o o oLiiliiltiliiliiJ 400 460 520 580 640 700 WAVELENGTH m }i Fig. 14. Spectral curves of cresyl violet. 1. National Aniline product. 2. Kresylecht violet from Bayer; 3. from Grubler. There is evidence that a stain satisfactory for one of these two latter purposes may not yield good results with the other. Wil- liams, for example, finds rather better results with the National
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Quinone-imine Dyes 123 H r I I -I \ Q 4-. CO o o oLiiliiltiliiliiJ 400 460 520 580 640 700 WAVELENGTH m }i Fig. 14. Spectral curves of cresyl violet. 1. National Aniline product. 2. Kresylecht violet from Bayer; 3. from Grubler. There is evidence that a stain satisfactory for one of these two latter purposes may not yield good results with the other. Wil- liams, for example, finds rather better results with the National Aniline product in biopsy work, while from other quarters com- plaints have been received concerning the same batch of this dye when used for making preparations from fixed tumor tissue, for which the imported dye is very satisfactory. There have been conflicting reports about the relative merits of domestic and foreign products for neurological work. Quite un- satisfactory results have been reported with the National Aniline product as a stain for Nissl granules; but Banny and Clark (1950) find that a cresyl violet just obtained from Coleman and Bell is satisfactory. Their finding has not been verified by others. Fur- thermore, the latter product is not the same as the foreign samples shown by graphs 2 and 3 in Fig. 14. It is believed to be a diethyl (instead of dimethyl) amino compound. It is plain that further work is necessary both on the chemistry of the imported product and on comparative value of the two types in staining. Experimental work on this dye has not pro-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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