. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Xanthene Dyes 197 r36 rose bengal C. I. NO. 779 Various shades denoted: Rose bengal extra^ SB N extra^ DYy B and 2B. I I NaO I I o_/\=o. C1~\/~C1 I CI C2oH205l4Cl4Na2; Mol. Wt. (An acid dye; absorption maximum S^^^^^-S) Solubility at 26°C: in water ; in alcohol The dye has a pleasing deep pink color; and although an acid dye it proves to have considerable affinity for bacterial protoplasm, and to have
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Xanthene Dyes 197 r36 rose bengal C. I. NO. 779 Various shades denoted: Rose bengal extra^ SB N extra^ DYy B and 2B. I I NaO I I o_/\=o. C1~\/~C1 I CI C2oH205l4Cl4Na2; Mol. Wt. (An acid dye; absorption maximum S^^^^^-S) Solubility at 26°C: in water ; in alcohol The dye has a pleasing deep pink color; and although an acid dye it proves to have considerable affinity for bacterial protoplasm, and to have good selective properties when used as a bacterial stain. It has been recommended (Conn 1918, 1921) for staining bacteria, especially in soil suspensions. It has also been used as a cytoplasm stain following hematoxylin or preceding toluidine blue. Maneval (1934!) employed it in the negative staining of bacteria and Ono (1934) for spirochaetes in blood. It also finds use in Delprat and Stowe's test (1931) for liver function. Popper (see Metcalf and Patton, 1944) mentions it as a useful fluorochrome in the study of fats under ultraviolet illumination. Smith and Daw- son (1944) employ this dye as a bacteriostatic agent in media de- signed to permit the growth of soil fungi while repressing the bacteria; this is the only instance yet noted of one of the fluoran derivatives being thus used. From Fig. 25, in which the spectral curve of this dye and phlox- ine B are compared, it will be seen that they differ more in shape than in the position of the absorption maximum. For technic of general staining of pure cultures of bacteria, see Staining Pro- cedures, p. IIIA2-5. For technic of Conn's stain for bacteria in soil, see Staining Procedures, 21. 4. PHENOLPHTHALEIN AND THE SULFONPHTHALEINS A phthalein is a compound of phthalic acid: COOH COOH. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - colorati
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