. Atlas and principles of bacteriology and text-book of special bacteriologic diagnosis. Bacteriology. SPOBOGENIG GRANULES. 21 looked upon as spores. (Compare Fig. 4, a, b, c.) In water, this shrinking rapidly disappears, and also under the prolonged action of saline solution. Migula (A. K., Bd. l), simultaneously and independently, reaches the same opinion as A. Fischer regarding the very large Bacillus oxal- atieus, a spore-forming variety related to the hay bacillus. It happens especially in this that in the pressing outward of the protoplasmic layer, the central fluid space becomes distinc
. Atlas and principles of bacteriology and text-book of special bacteriologic diagnosis. Bacteriology. SPOBOGENIG GRANULES. 21 looked upon as spores. (Compare Fig. 4, a, b, c.) In water, this shrinking rapidly disappears, and also under the prolonged action of saline solution. Migula (A. K., Bd. l), simultaneously and independently, reaches the same opinion as A. Fischer regarding the very large Bacillus oxal- atieus, a spore-forming variety related to the hay bacillus. It happens especially in this that in the pressing outward of the protoplasmic layer, the central fluid space becomes distinct; in dehydrating m'edia it becomes syialhr; in water, larger. While the botanists have hitherto sought in vain for a true nucleus, Arthur Meyer would recognize it in small, single, oval granules, staining with Ruthenium red and potassium iodid (Flora, 1897, Band 84, 185). Compare. ^ a i c a, Spirillum undula. b, Bacillus Solmsii. c. Vibrio cholerse. Fig. 4.—Plasmolysis (after A. Fischer). also the hitherto scarcely studied observations of (C. B. XXIII, 433), according to which nuclei were easily stained by primulin and hot Bordeaux red. In the interior of bacterial cells there are found, after proper staining, very many varieties of peculiar granules, which Babes, who discovered them, named metachromatic bodies (i. e., staining differently from the cell-body). Ernst, the first accurate investigator of these bodies, called them nuclei or sporogenic granules. While I refer to the literature of Bab^s (Z. H. XX, 412), which is rich in controversy, I give only the seductively clear view of one of the latest investigators of the subject, Bunge (Fort, der Med., XIII, 813 and 853) distinguishes : 1. Er}Vii'.f granules. They stain blackish-blue when treated with warm Loffler's methylene-blue, washed in water, and after-stained with Bismarck brown. These granules are entirely absent in many. Please note that these images are extracted from scanned page images that ma
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