. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Quinone-imine Dyes 127. O tTi I I I I I I I I rNo I I I J *'400 460 520 580 640 700 WAVELENGTH Tn >x Fig. 15. Spectral curves of two azin dyes: 1. Neutral red. 2. Azocarmine G. It has special value where a weakly basic, non-toxic dye is called for, as in vital staining. It is used for staining living pro- tozoa, and as a vital stain for nuclei in tissue; also for the 'Vital" staining of blood, that is of fresh blood, obse
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Quinone-imine Dyes 127. O tTi I I I I I I I I rNo I I I J *'400 460 520 580 640 700 WAVELENGTH Tn >x Fig. 15. Spectral curves of two azin dyes: 1. Neutral red. 2. Azocarmine G. It has special value where a weakly basic, non-toxic dye is called for, as in vital staining. It is used for staining living pro- tozoa, and as a vital stain for nuclei in tissue; also for the 'Vital" staining of blood, that is of fresh blood, observed under a micro- scope in a moist chamber, and for staining fresh gonorrhoeal pus under similar conditions. It proves useful as an indicator of re- action of the contents of living plant cells. The chief draw-back to neutral red in vital staining is the toxicity of certain lots that have been on the market. This toxicity seems to be due to impurities present in the dye. Phillips and Cohen (1927) have, in fact, shown that these impurities can be eliminated more readily by preparing the dye as an iodide instead of a chloride. Their process, however, has not been adopted by manufacturers as yet, because of technical difficulties. Nevertheless the recent samples of neu- tral red chloride submitted for certification have been so well purified that they seem to be free from toxicity. For technic of supravital staining of blood, with Janus green, see Staining Pro- cedures, p. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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