. Journal of agricultural research . 2-hour ° growth on potato agar, stained by Duck- with rOUUdcd Cnds and OCCUrS USUally walls method to show flageiia. X gingly or in pairs. When stained from2-day-old potato agar cultures with Ziehls carbol fuchsin or gentianviolet, the cells average about long by i .2m wide. When stained withDuckwalls flagellum stain the average measurement is about 3m by Zettnows and Duckwalls flagellum stains have shown the organ-ism to be motile by means of one to several polar flagella (fig. i).No endospores or involution forms have been observed


. Journal of agricultural research . 2-hour ° growth on potato agar, stained by Duck- with rOUUdcd Cnds and OCCUrS USUally walls method to show flageiia. X gingly or in pairs. When stained from2-day-old potato agar cultures with Ziehls carbol fuchsin or gentianviolet, the cells average about long by i .2m wide. When stained withDuckwalls flagellum stain the average measurement is about 3m by Zettnows and Duckwalls flagellum stains have shown the organ-ism to be motile by means of one to several polar flagella (fig. i).No endospores or involution forms have been observed. Capsules werenot demonstrated when stains were made from potato or beef-peptoneagar cultures, but well-developed capsules were found present on bloodagar. Both Welchs and Hisss methods of staining were used. Lightflocculent and sometimes membranous pellicles developed on the surfaceof certain liquid media such as bouillons of favorable reactions, sugarsolutions, and on Fermis and Uchinskys solutions. The organism isgram Nov. Bacterial Blight of Soybean 183 CULTURAIv CHARACTERS Unless otherwise specified, all cultures were incubated at 25° C, a tem-perature very favorable for the growth of the organism. Reactions weredetermined by titration with phenolphthalein as indicator, the trial solu-tion always having been boiled previous to test. All references to reac-tion of media are recorded in terms of Fullers scale. Ridgways colorstandards * were used in the determination of color. Agar poured plates.—On potato agar, colonies appeared in about48 hours and at the end of 5 days were from 2 to 5 mm. in were creamy white tinged with brown, circular, shining, and con-vex with umbonate center. The margin in general was entire, althoughit may be slightly lobed. The surface may show indication of irregularwrinkling or beading in the central part, or there may be more or lessconcentric and radiating convolutions throughout the colony with adefinite gro


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Keywords: ., bookcentury1900, bookdecade1910, booksubjectagricul, bookyear1913