. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 148 Biological Stains CTI3. C21H22N3CI; Mol. Wt. {A basic dye; absorption maximum about 55J/) n5 New Fuchsin (Magenta III) c. i. no. 678 Synonyms: Isorubin. Fuchsin NB. This compound is tri-methyl fuchsin, or triamino-tritolyl- methane chloride: CH3 I H CI /""\ NH. N_/~\_C _. „ ~\_NH2 H I \^/ CH3 I CHa C22H24N3CI; Mol. Wt. {A basic dye; absorption maximum about 556) Solubility at 26°C: in water ; in
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 148 Biological Stains CTI3. C21H22N3CI; Mol. Wt. {A basic dye; absorption maximum about 55J/) n5 New Fuchsin (Magenta III) c. i. no. 678 Synonyms: Isorubin. Fuchsin NB. This compound is tri-methyl fuchsin, or triamino-tritolyl- methane chloride: CH3 I H CI /""\ NH. N_/~\_C _. „ ~\_NH2 H I \^/ CH3 I CHa C22H24N3CI; Mol. Wt. {A basic dye; absorption maximum about 556) Solubility at 26°C: in water ; in alcohol From the general rule concerning alkyl substitution it will be understood that new fuchsin is the deepest in shade of these four dyes, pararosanilin the least so. The commercial fuchsins, there- fore, which consist of mixtures of the first three vary in depth ac- cording to the proportions in which they are present. A typical spectrophotometric curve of rosanilin (Magenta I) is given in Fig. 20. Those for Magentas O, II, and III are not given here, as they are so nearly the same that they can be dis- tinguished only by careful measurement, and the differences would not show in such a graph as those here included. It has proved from the investigations of the Stain Commission that not all basic fuchsins furnished as biological stains are mix- tures of these three compounds. Many of the samples prove to be fairly pure pararosanilin; while others are deeper in shade than rosanilin and presumably contain appreciable quantities of magenta II. All of these dyes differ so very slightly from each. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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