. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. STAINING OF PYCNOTIC NUCLEI PLATE I " ** ;""" f* 1 ",. * rc A 3a • 4a I\ » P V A * 3b 4b 1. Nuclear histone staining (fast green at pH 8) of normal follicle cells sur- rounding a young oocyte. Formalin, 10 i", X 570. FIGURE 2. Nuclear histone staining of pycnotic masses in the antrum of an old follicle. Formalin, 10 M, X 570. FIGURES 3 AND 4. Distribution of Millon-protein (a) and Feulgen-DNA (b) in the same follicle cells. Carney, 3 M, X 1100. Figures 3a and b pycnotic nuclei; fig
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. STAINING OF PYCNOTIC NUCLEI PLATE I " ** ;""" f* 1 ",. * rc A 3a • 4a I\ » P V A * 3b 4b 1. Nuclear histone staining (fast green at pH 8) of normal follicle cells sur- rounding a young oocyte. Formalin, 10 i", X 570. FIGURE 2. Nuclear histone staining of pycnotic masses in the antrum of an old follicle. Formalin, 10 M, X 570. FIGURES 3 AND 4. Distribution of Millon-protein (a) and Feulgen-DNA (b) in the same follicle cells. Carney, 3 M, X 1100. Figures 3a and b pycnotic nuclei; figures 4a and b normal cells at the left, a degenerating cell at the right. Compare identical nuclei, as identified by arrows, in 3a and 3b. (Photomicrographs by Dr. Walter Plant.) come pycnotic by uniform shrinkage without fragmentation and losr none- of their DNA during a period of 24 hours autolysis (Roslansky and Alfert. unpublished). In Table II the Feulgen/fast green and Feulgen/methyl green ratios of the formalin series are given in the first two columns. These data indicate a striking change in the fast green stainability of histone and a small change in the methyl green stainability of DNA. The ratios are lowered, because of a relative increase in acidophilia of histone, and in basophilia of DNA. Put in other terms, there seems to be more histone and more methyl green stainable DXA in the pycnotic nuclei, per unit Feulgen-DNA content, than was present in the normal nuclei from which they were derived. The methyl green effect is exactly opposite to that described by Letichtenberger (1950) for pycnotic tumor nuclei: those had an increased Keulgen /methyl green. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole,
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
