. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 188 Biological Stains assumed that this dye is not soluble in alcohol. This is not true, however. A typical absorption curve of eosin Y is given in graph 2, Fig. 24, in which it is compared with the similar spectral data for eosin B and ethyl eosin. Like fluorescein, eosin Y shows greenish yellow fluorescence, especially in alcoholic solution. This fluorescence is visible in .6- > H (/) Z UJ o O -I o .1- 400. .3- 42
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 188 Biological Stains assumed that this dye is not soluble in alcohol. This is not true, however. A typical absorption curve of eosin Y is given in graph 2, Fig. 24, in which it is compared with the similar spectral data for eosin B and ethyl eosin. Like fluorescein, eosin Y shows greenish yellow fluorescence, especially in alcoholic solution. This fluorescence is visible in .6- > H (/) Z UJ o O -I o .1- 400. .3- 420 440 460 480 500 520 540 )" Fig. 24. Spectral curves of three eosins: Eosin B. 2. Eosin Y. 3. Ethyl eosin. ordinary light, but although quite strong, "is not so pronounced nor visible in such great dilution as in the case of fluorescein. It is interesting that in this series of fluoran derivatives, tinctorial power increases but fluorescence decreases with the ascending members of the series; thus the erythrosins are less fluorescent than eosin, while phloxine and rose bengal show scarcely any fluorescence in ordinary light. In spite of the marked fluorescence of eosin Y, it does not seem to have been suggested as a Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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