. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LARVAL METAMORPHOSIS OF STROMBUS GIGAS 135 v> o o E Q) C 01 Concentration (ul extract/ml) Figure 1. Percent metamorphosis of queen conch larvae in response to specific concentrations of Laurencia poitei extract. Points are means ± SD; n = 5. after the larvae contacted the bottom. After 30 min, all larvae were on the bottom and their lobes showed in- creased curling; after 90 min, individual cilia and portions of the velar lobes began to drop off. Cilia on the isolated lobes continued to beat for at least 10 min. At 11
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LARVAL METAMORPHOSIS OF STROMBUS GIGAS 135 v> o o E Q) C 01 Concentration (ul extract/ml) Figure 1. Percent metamorphosis of queen conch larvae in response to specific concentrations of Laurencia poitei extract. Points are means ± SD; n = 5. after the larvae contacted the bottom. After 30 min, all larvae were on the bottom and their lobes showed in- creased curling; after 90 min, individual cilia and portions of the velar lobes began to drop off. Cilia on the isolated lobes continued to beat for at least 10 min. At 110 min, some of the larvae had completed metamorphosis. At this time, many still retained remnants of their velar lobes, but they had begun using the foot to crawl on the bottom of the containers. By 2 h, more than 50% of the larvae had undergone complete metamorphosis. Amino acid composition of the algal extract Of the 17 amino acids detected in the hydrolyzed w ^^^ 1 .!£ '« 80 — _ o C J a. 70 - V y X o E 60 — ; < m 50 — / _ a! / S 40 — B / / c 0) 30 — [/ u ( 01 20 — ; \ Q. / 10 — V 0 ( i c 3 J 1 : 1 1 J 3 4 i 1 i Duratlc )n of ex 30sure (h) Figure 2. Percent metamorphosis of queen conch larvae in response to seawater only (sw. a negative control) for 5h and an extract of Laurencia poitei (20/j1 extract/ml seawater) for , 1. 2, 3, and 5 h. Points are means ± SD; n = 5. Data points with the same letter above the error bar are not significantly different at P s Table I Coitceinratiiiii of umiiio aculs detected in the liydrolyzed less than I i<Da fraction of an ac/neous Laurencia poitei extract Compound Glutamine/Glutamate Glycine Alanine Asparagine/Aspartate Serine Threonine Honioserine Valine 4-Hydroxyproline Histidine Leucine Isoleucine Hydroxylysine Lysine Arginine Phenylalanine Methionine Concentration ifiM) 46 33 19 18 14 12 Only amino acids detected at concentrations greater than 1 ^JM are
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