. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 128 Biological Stains l8 NEUTRAL VIOLET CH3 \ C. I. NO. CH3 N N. N CHj J CIIj \/-N=\y NH-\/ C22H25N6CI; Mol. Wt. {A weakly basic dye; absorption maximum 533) Solubility at 26°C: in water ; in alcohol This dye is very similar in its properties to neutral red, except that, due to its greater molecular weight, it is more bluish, giving a violet instead of a red color. It can be used as an indicator, bu
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 128 Biological Stains l8 NEUTRAL VIOLET CH3 \ C. I. NO. CH3 N N. N CHj J CIIj \/-N=\y NH-\/ C22H25N6CI; Mol. Wt. {A weakly basic dye; absorption maximum 533) Solubility at 26°C: in water ; in alcohol This dye is very similar in its properties to neutral red, except that, due to its greater molecular weight, it is more bluish, giving a violet instead of a red color. It can be used as an indicator, but has been seldom used in histology. Unna (1921) however, has used it in a dye mixture employed in the study of chromolysis. b. Safranins Quite a long series of azin dyes are known in which one of the nitrogen atoms of the azin group is pentavalent and another ben- zene ring is attached to it. This pentavalent nitrogen allows the compounds to behave like ammonium bases; so with the amino groups which are always present, the basic properties of these dyes are very strong. The theoretical base of the simplest safranin would have the formula: HaN This form of ammonium base does not actually exist, as the safra- nin bases really occur in the form of anhydrides; but salts of these ammonium bases are the commonly known dyes. The com- mercial dyes are ordinarily chlorides. There are two groups of safranins: the benzo-safranins in which the azin group unites two benzene rings; and the naphtho-safranins in which it unites two naphthalene groups. The simplest safranin is pheno-safranin, which is the chloride of the theoretical base just given, namely: PHENO-SAFRANIN C. I. NO. 840 Synonym: Safranin B extra. /\ N_/\. Lis H,N / \ NH, /\ CI 0 C18H15N4CI; Mol. Wt. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original C
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