. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . es methylene-blue solutions, or with carbol-33 514 - SPIROCHETA AND ALLIES fuchsin; best with the Romanowsky method or its modifications. Thejare negative to Gram. Biological Characters.—In fresh preparations from the blood the spirechetes exhibit active progressive movements, accompanied by verjrapid rotation in the long axis of the spiral filaments or by undulating movements. They are found only in the blood or blood organsnever in the secretions, and only during the fever, not in the intermissio
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . es methylene-blue solutions, or with carbol-33 514 - SPIROCHETA AND ALLIES fuchsin; best with the Romanowsky method or its modifications. Thejare negative to Gram. Biological Characters.—In fresh preparations from the blood the spirechetes exhibit active progressive movements, accompanied by verjrapid rotation in the long axis of the spiral filaments or by undulating movements. They are found only in the blood or blood organsnever in the secretions, and only during the fever, not in the intermissions, or at most singly at the beginning of, or for a short time after, arattack. When kept in blood serum, or a per cent, solution • of sodiunchloride, they continue to exhibit active movements for a considerabktime. They may be preserved alive and active for many days in sealectubes. They are killed quickly at 60° C, but they remain alive foisome time at 0° C. Unsuccessful efforts to cultivate them in artificiaculture media have been made from time to time. Koch has observed f\if\^. Fig. 175.—Photograph of Sp. obermeieri Fig. 176.—Spiroclieta ohermeieri blood showing terminal flagellum. X 3000 diam- smear. Fuchsin. X 1000 diameters. (Froneters. (After Novy.) Itzerott and Niemann.) an increase in the length of the spirilla and the formation of a tangled masiof filaments. Novy finally succeeded in cultivating them in celloidin capsules placed in the peritoneum of rats. His culture remained viruleni(with a slight loss) for many generations. Noguchi has also cultured themPathogenesis.—In man, whether the disease is acquired naturally oiby artificial inoculation, the organism causes the following symptomsAfter a short period of incubation the temperature rises rapidly, remainihigh for five to seven days, and then returns to normal by crisis. Abouseven days later there is another sudden rise of temperature, but thiitime the crisis occurs sooner. A second or third rel
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