. Special pathology and therapeutics of the diseases of domestic animals. Veterinary medicine. Fig. 77. Bac. Tuberculosis, acid- fast form. Bronchial secretion from cattle. Stained by Ziehl- Nelssen's method. which. Spengler refers to as "splinters" and regards as true spores,. 'Most investigators, however, look upon these bodies as chromatin granules such as are found also in other bacteria whether they are sporulating or non-sporulating species. Staining. The typical tubercle ba- cillus is stained most readily and charac- teristically with a warm carbol-fuchsin solution or with any
. Special pathology and therapeutics of the diseases of domestic animals. Veterinary medicine. Fig. 77. Bac. Tuberculosis, acid- fast form. Bronchial secretion from cattle. Stained by Ziehl- Nelssen's method. which. Spengler refers to as "splinters" and regards as true spores,. 'Most investigators, however, look upon these bodies as chromatin granules such as are found also in other bacteria whether they are sporulating or non-sporulating species. Staining. The typical tubercle ba- cillus is stained most readily and charac- teristically with a warm carbol-fuchsin solution or with water gen- tian-violet. After once absorbing the stain the latter becomes so well fixed that it is impossible to remove it with alcohol or mineral acid (5% sulphuric acid— 30% nitric acid). This characteristic (acid-fast or alcohol-fast) which is met also in the bacillus of leprosy and in sev- eral saprophytic bacteria, is due to the presence of. fatty acids in the body of the bacillus (Deycke). No other bacteria ex- cept those mentioned possess this charac- teristic. Much's granular form of the tubercle bacillus may be stained by Gram's method of subjecting it to the action of aniline- gentian violet for 24 to 48 hours at body temperature. (See Diagnosis.) Culture. The tubercle bacilli will grow only in the presence of oxygen and at a temperature varying from 29 to 42° C, its growth being most prolific at blood temperature. The best culture media are those containing egg albumen, although the bacillus will grow also on common laboratory media which do not contain this substance. The chemical reaction of the medium must be neutral or slightly acid. The presence of glycerin, grape sugar or phosphoric acid favors its growth which is Usually very slow. Cultures are never visible before the end of a period of at least one or two weeks. On blood serum and on 3 to 5% glycerin agar the cultures appear as fol- lows: In the course of two weeks after inoculation of material s
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