. Effects of pollutants on marine organisms : deliberations and recommendations of the NSF/IDOE Effects of Pollutants on Marine Organisms Workshop, held in Sidney, British Columbia, August 11-14, 1974 . 2 ^ 6 8 10 12 U 16 18 20 22 lU Time(min) Figure 2. Effect of water solubles from No. 2 fuel oil on photosynthesis of 3 micro- algae: Thalassiosira pseudonana (3H), Agmenellum quadruplicatum (PR-6). and Chlorella auiotrophica (580). Conditions: No. 2 fuel oil equilibrated for 24th stirring with filtered seawater; seawater separated and filtered through iim Millipore filter and added to alga
. Effects of pollutants on marine organisms : deliberations and recommendations of the NSF/IDOE Effects of Pollutants on Marine Organisms Workshop, held in Sidney, British Columbia, August 11-14, 1974 . 2 ^ 6 8 10 12 U 16 18 20 22 lU Time(min) Figure 2. Effect of water solubles from No. 2 fuel oil on photosynthesis of 3 micro- algae: Thalassiosira pseudonana (3H), Agmenellum quadruplicatum (PR-6). and Chlorella auiotrophica (580). Conditions: No. 2 fuel oil equilibrated for 24th stirring with filtered seawater; seawater separated and filtered through iim Millipore filter and added to algal suspension 8 min before light turned on. Concentration of this water soluble fraction is given in percent, v/v ( = ml of algal sus- pension plus ml of seawater-oil solubles). Controls: seawater plus medium ASP-2. Algal strains are identified in Table 2. The algal concentrations for PR-6, 3H and for 580 are 1 X 10' cells/ml in medium ASP-2. Electrode current at air saturation, medium ASP-2 = fia. Light intensity, limited by Baird-Atomic Hot Mirror (34-01-2) to 350 to 800 nm, was 580 mW/cm^ at level of electrode chamber, measurement temperature 30°C. With both the sea water equilibrated with fuel oil and the crude oils, the toxic activity is mainly localized in medium and higher boil- ing fractions derived from distillation cuts from these materials. The question of the toxicity of fuel oils to the microalgae has been examined in greater detail. Four fuel oil samples were obtained from Exxon Corporation. Approximately 50% of the compounds in the water solubles from these four fuel oils have been identified via gas chromatography and mass spectrometry. In addition to the well de- scribed types of compounds (naphthalenes and benzenes) expected in water soluable extracts phenols, anilines, and indoles were found 16
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