. Bacteriological methods in food and drugs laboratories : with an introduction to micro-analytical methods . Bacteriology; Food; Drugs. THE TEST FOR THE BACILLUS OF CHOLERA II3 endes to locate in the bile duct or gall-gladder, where it may re- main dormant for a long period of time. This observation has led to the use of bile as an enriching medium for both organisms. The cholera vibrio work in the food and drug laboratory may resolve itself into the isolation of the germ from water supplies, from vegetables and possibly from feces and from sewage, and consists in the use of special culture m


. Bacteriological methods in food and drugs laboratories : with an introduction to micro-analytical methods . Bacteriology; Food; Drugs. THE TEST FOR THE BACILLUS OF CHOLERA II3 endes to locate in the bile duct or gall-gladder, where it may re- main dormant for a long period of time. This observation has led to the use of bile as an enriching medium for both organisms. The cholera vibrio work in the food and drug laboratory may resolve itself into the isolation of the germ from water supplies, from vegetables and possibly from feces and from sewage, and consists in the use of special culture media, special cultural methods, inoculation methods and agglutination tests. It is interesting to note that the method now in use for isolating the cholera vibrio from water supplies is the original Koch method, done as follows. Add I per cent, each of peptone and salt to loo cc. of the suspected water and incubate at 38° C. Examine microscopically at inter- vals of 8, 12 and 18 hr. As soon as curved and comma-shaped organisms appear, plate on agar and make such additional tests as may be necessary to prove the Fig. cWera showing invo- . ,, , , , lutiou forms (X 1000).—(MacNeal, presence of the cholera germ, such after Van Emengen.) as the nitroso reaction, agglutina- tion test, Pfeiffer's phenomenon, etc. It is not practical to enter into a fuller discussion of the subject. More complete details will be found in the works on bacteriology and in bulletins and reports on bacteriology and on hygiene. For example, the U. S. Public Health Service has worked out a quick routine method for isolat- ing the cholera germ from feces, used in the U. S. Quarantine Ser- vice and at the quarantine station of New York, as reported in the Journ. of the Am. Pub. Health Association (Dec, 1911) and a condensed summary of the general methods may be found in the admirable little work by Stitt (Practical Bacteriology, Blood Work and Parasitology, 1913). Numerous special reports will be fou


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