. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 286 M. W. VANDERSEA ET Figure 3. At stage 37. dorsal views of control larvae (A) and larvae exposed at gastrulation to low conceiitrationsof retinoic acid (RA)—10""'A/(B). XO'" M (C). 10"* M (D). and 10 ' M (E) —show well- developed eyes (arrows. A and E) and pectoral fins (arrowheads, A and E). Larvae exposed at gastrulation to lO^^MRA (F) show anterior and ventral rotation of the eyes (arrows), normal Hn development (arrowheads), pencardial edema (pe). and shortened trunks and curled tails (asteris
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 286 M. W. VANDERSEA ET Figure 3. At stage 37. dorsal views of control larvae (A) and larvae exposed at gastrulation to low conceiitrationsof retinoic acid (RA)—10""'A/(B). XO'" M (C). 10"* M (D). and 10 ' M (E) —show well- developed eyes (arrows. A and E) and pectoral fins (arrowheads, A and E). Larvae exposed at gastrulation to lO^^MRA (F) show anterior and ventral rotation of the eyes (arrows), normal Hn development (arrowheads), pencardial edema (pe). and shortened trunks and curled tails (asterisk. F). At \0^' M RA. larvae display multiple pectoral hns (arrowheads. G). a heavily pigmented trunk (tr). and curled tail (asterisk. G). A lateral view of a larva treated at lO""* M RA (H) shows a shortened trunk (tr) atop the yolk. Anterior is to the top (A-G) and to the left (H). Scale bar = 200 /jm. cells are pooled around the urinary bladder, and muscular contractions occur in the trunk, but not as frequently as in the control embryos. 1 X lO"" M RA. Embryos exposed to 10"^ M RA (Fig. 2H) lack obvious head structures and appear only as a thin trunk (tr) set on an expanded visceral cavity and gut. Pectoral Hns are absent. The urinary bladder is surrounded by blood cells at the base of the trunk where the bladder meets the yolk sac (not shown). Muscular movements occur in some embryos, and a small, stubby tail projects from the trunk (not shown). Stage 37: Multiple fin phenotypes 1 X 10^'' M RA. At stage 37, controls and larvae that have been exposed as gastrulae to 10 '" to 10^^ M RA are normal in appearance (Fig. 3A-E) and can be induced to hatch at 10 days post-fertilization. Embryos exposed to greater concentrations of RA do not hatch but survive for an extended period. These larvae are smaller and retain a large volume of yolk (compare Fig. 3A-E with 3F- H). Embryos exposed to 10 ''M RA develop into larvae with smaller than normal eyes
Size: 1845px × 1355px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauth, bookcentury1900, booksubjectbiology, booksubjectzoology
