. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 54 ALFRED B. CHART TABLE III Assay of nerves obtained from pre- and post-shed Patina miniata. Figures expressed as shedding index. Lyophilized Nerve Extract (in sea water) 0 5 10 25 100 Pre-shed (ripe) Post-shed (unripe) that inhibited shedding of ovarian fragments even though all fragments were under the influence of active purified shedding substance (#3). Since this fraction in- hibited shedding activity, it was called "; Similar ass
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 54 ALFRED B. CHART TABLE III Assay of nerves obtained from pre- and post-shed Patina miniata. Figures expressed as shedding index. Lyophilized Nerve Extract (in sea water) 0 5 10 25 100 Pre-shed (ripe) Post-shed (unripe) that inhibited shedding of ovarian fragments even though all fragments were under the influence of active purified shedding substance (#3). Since this fraction in- hibited shedding activity, it was called "; Similar assays were per- formed using extremely large ovaries from maximally ripe starfish (Patiria miniata). Such ovarian fragments, provided that they were taken from very ripe females, shed spontaneously (even in the absence of added shedding substance) within 15 to 30 minutes after immersion in sea water. However, when these fragments were immersed in each of the above fractions taken from Sephadex columns, all fractions allowed shedding to occur except that fraction (#5) previously found to contain shedhibin. No shedhibin could be detected in those radial nerves taken from post- shed, unripe females whose crude nerve extract induced shedding at higher con- .00 + 3 SW. + FRACTION MIXTURE FIGURE 7. Graphic representation of Patiria miniata ovarian fragment assay, illustrating presence of shedhibin in fraction #5 of fractionated Patiria miniata radial nerves. Five-mi, fractions eluted from short Sephadex G-25 column were assayed after mixing with an equal volume of fraction #3, known to contain shedding Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lil
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