Electron microscopy; proceedings of the Electron microscopy; proceedings of the Stockholm Conference, September, 1956 electronmicrosco00euro Year: 1957 232 M. K. KEECH AND R. REED •.^J^ Fig. 3. 'Moth-eaten' fibre disintegrating into an 'elastin network' (MEFC). Obtained after heating the MEF prepara- tion from a 9-year-old child in water for 1 hour at 55°C and a further hour at 75''C. Magnification 11,000. Fig. 4. Another example of a 'moth-eaten' fibre conversion structure (MEFC) from the same preparation as fig. 3. Magnification 11,000. The manufactured networks (MN) were found only in t
Electron microscopy; proceedings of the Electron microscopy; proceedings of the Stockholm Conference, September, 1956 electronmicrosco00euro Year: 1957 232 M. K. KEECH AND R. REED •.^J^ Fig. 3. 'Moth-eaten' fibre disintegrating into an 'elastin network' (MEFC). Obtained after heating the MEF prepara- tion from a 9-year-old child in water for 1 hour at 55°C and a further hour at 75''C. Magnification 11,000. Fig. 4. Another example of a 'moth-eaten' fibre conversion structure (MEFC) from the same preparation as fig. 3. Magnification 11,000. The manufactured networks (MN) were found only in the presence of MEFC and probably repre- sent the complete conversion or separated portions of these structures. Similar MN occurred after heating 'alkali-produced elastin'. Collagen was seen only in the adult specimens and exhibited slight 'standard' collagenase change. 3. Effect of other reagents on MEF. A dramatic change from 90 %%%%%%%%%%%%%%%% MEF to 85-90 %%%%%%%%%%%%%%%% large 'elastic networks' was produced by 1 %%%%%%%%%%%%%%%% sodium periodate (in buffer pH ) and by elastase (acting at its op- timum pH of ). Also numerous large and small dense bits were noted, either converting into 'elas- tin' individually or situated in groups linked together by 'elastin'. A more prolonged incubation with periodate (3 hrs. at 37°C) caused disappearance of the MEF and elastic structures, but numerous small, round, dense balls were found, similar to those described by Hall et al. (4) as a product of the action of elastase on elastin. Incubation of the starting material in borate buffer pH alone did not pro- duce any change, but prolongation of incubation with elastase caused rapid digestion of the 'elastic networks'. Two per cent acetic acid at room temperature produced no macroscopic change but under the electron microscope the dense bits were broken up into smaller pieces and MEFC and MN were seen. After 1 hour at lOO'C the dense bits were markedly decreased, accompanie
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