. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. a Cyanobacteria - small strain • Cyanobacteria - ACMM326 „ 3-, EarlV brachiolaria 01. FLC : living cells In media Figure 2. Comparison of the proportion of heat-killed, fluorescence- labeled cells to living cells between experimental media and the gut con- tent of the larvae of Acanthoster plonci. Direct count of algal cells in the gut of echinoderm larvae is not new (Strathmann, 1971), but has been limited by the resolution of light microscopy. The use of an epi- fluorescence microscope in feeding studies promises more reli
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. a Cyanobacteria - small strain • Cyanobacteria - ACMM326 „ 3-, EarlV brachiolaria 01. FLC : living cells In media Figure 2. Comparison of the proportion of heat-killed, fluorescence- labeled cells to living cells between experimental media and the gut con- tent of the larvae of Acanthoster plonci. Direct count of algal cells in the gut of echinoderm larvae is not new (Strathmann, 1971), but has been limited by the resolution of light microscopy. The use of an epi- fluorescence microscope in feeding studies promises more reliable data with less effort and time. The autofluores- cence of algal cells, if samples are examined immediately after fixation, can be seen in the gut. DTAF-labeled cells produce bright yellow-green fluorescence at the broad blue excitation band (450-490 nm) of the Zeiss filter set used. The autofluorescence of two strains of phycoerythrin-rich cyanobacteria is orange-yellow and more or less similar to DTAF fluorescence. Nevertheless, the DTAF fluores- cence can be distinguished easily from the autofluores- cence of cyanobacteria, unless the gut becomes congested with cells. Selective ingestion of FLC over living cells of Tetra- selmis sp. by COTS larvae may be ascribed to the ineffi- cient capturing of the motile cells of Tetraselmis sp. Al- ternatively, living cells of Tetraselmis sp. may release chemical compounds that COTS larvae do not favor. The possibility of such chemosensory feeding has been sug-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marin
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
